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Image Search Results
Journal: Cell Death & Disease
Article Title: The DNA methylation-regulated miR-193a-3p dictates the multi-chemoresistance of bladder cancer via repression of SRSF2/PLAU/HIC2 expression
doi: 10.1038/cddis.2014.367
Figure Lengend Snippet: The effect of both miR-193a and its target genes on the BCa chemoresistance. ( a ) The experimental scheme. ( b ) The relative cell survival by the IC 50 -dosed indicated drugs in the miR-193a-3p (3PM) or miR-193a-5p (5PM) mimic versus the mock (NC) of 5637 cells in plot. ( c ) The relative level of both SRSF2 (-3p target) and TP73 (-5p target) normalized with the GAPDH level, in the same set of 5637 cells in panel ( b ). ( d ) The relative cell survival by the IC 50 -dosed indicated drugs in miR-193a-3p (3PA) or miR-193a-5p (5PA) antagomiR versus the mock (NC) in H-bc cells in plot. ( e ), The relative level of SRSF2 (-3p target) and TP73 (-5p target) proteins normalized with the GAPDH level, in the same set of H-bc cells in panel ( d ). ( f ) The relative cell survival of the siRNA or 3PM-transfected or NC-transfected 5637 cells under the IC 50 -dosed Pa and Ad, respectively. ( g ) The protein level of the downstream genes in the same set of cells in panel ( f ), by western blotting analysis. ( h ) The relative cell survival of 5637 cells transfected with the indicated transfection under the IC 50 -dosed Pa and Ad, respectively. ( i ) The protein level of the experiment in panel ( h ) (Pa-treated group), by western blotting analysis. ( j ) The summary of the effects on the cell survival of the IC 50 -dosed drug-trigger cell death by miR-193a-3p's target genes. ( k ) The relative cell survival under the treatment of the IC 50 -dosed Pa and Ad in H-bc cells transfected with the SRSF2-GFP, PLAU-GFP expression constructs, 3PA or NC, respectively. (* P <0.05; ** P <0.01)
Article Snippet: Briefly, the cells were transfected in triplet with each firefly luciferase reporter construct in combination with the Renilla luciferase construct using
Techniques: Transfection, Western Blot, Expressing, Construct